Latest updates for Long Read Sequencing

Fresh curated links around long read sequencing are collected here so marketers can spot useful updates and turn timely ideas into posts faster.

Recent items include:

  • Short-read RNA-seq yields lower estimates of A-to-I RNA editing levels than long-read cDNA sequencing
  • Eclipsebio expands its RNA characterization capabilities with multi-attribute nanopore-based sequencing assay eSTRAND RN
  • Long-read RNA sequencing reveals how neurons choose alternative RNA endings

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rna-seqblog.com /1 month ago

Short-read RNA-seq yields lower estimates of A-to-I RNA editing levels than long-read cDNA sequencing

RNA sequencing comparisons show that short reads can underestimate A-to-I RNA editing, while long-read sequencing provides more accurate measurements through improved genomic align...

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rna-seqblog.com /1 month ago

Eclipsebio expands its RNA characterization capabilities with multi-attribute nanopore-based sequencing assay eSTRAND RN...

RNA sequencing with eSTRAND RNA QC enables simultaneous measurement of RNA identity, poly(A) tail length, capping efficiency, fragmentation hotspots, and dsRNA...

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rna-seqblog.com /3 weeks ago

Long-read RNA sequencing reveals how neurons choose alternative RNA endings

Long-read RNA sequencing and APALORD reveal how alternative polyadenylation changes during neuronal differentiation and identify new patterns of RNA processing across species...

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rna-seqblog.com /1 month ago

Eclipsebio expands its RNA characterization capabilities with multi-attribute nanopore-based sequencing assay eSTRAND RN...

Eclipsebio’s eSTRAND RNA QC uses direct RNA sequencing to measure multiple RNA quality attributes, including identity, poly(A) tails...

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rna-seqblog.com /1 month ago

An end-to-end computational framework for “Record-seq” transcriptional recording data

A new framework adapts RNA sequencing analysis for Record-seq, improving spacer extraction, transcription-unit quantification, normalization, differential expression, and modeling...

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rna-seqblog.com /2 weeks ago

How should biological replicates be handled in long-read RNA sequencing?

Long-read RNA sequencing analysis shows that handling biological replicates differently can influence transcript reconstruction and the discovery of novel RNA isoforms...

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rna-seqblog.com /1 day ago

Recordanelles enable RNA sequencing to capture a history of gene activity in living cells

Researchers developed recordanelles that preserve gene expression histories for days, allowing single-cell RNA sequencing to detect transient cellular responses that conventional m...

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genengnews.com /2 weeks ago

Expanding the Possibilities of Next-Generation Sequencing

Sequencing by expansion (SBX) and Roche’s AXELIOS 1 platform introduce a new approach to single-molecule sequencing, designed to give researchers flexibility across sequencing work...

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journals.plos.org /1 month ago

Direct microhaplotype genotyping for GT-seq (Genotyping-in-Thousands by Sequencing) using a diploid abundance model

by Nathan R. Campbell, Amanda R. Campbell, Shannon K. Blair, Amanda J. Finger GT-seq (Genotyping-in-Thousands by Sequencing) is widely used for high-throughput amplicon genotyping...

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rna-seqblog.com /1 month ago

Ultrafast and reference-free sequence discovery in single-cell data

Malva enables ultrafast, reference-free searches across massive single-cell RNA sequencing datasets for mutations, splice junctions, pathogens, transcripts, and other sequence-leve...

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rna-seqblog.com /3 weeks ago

The Human RNome Project Consortium seeks to publish first-ever complete sequence of the body’s RNA

RNA sequencing efforts from the Human RNome Project aim to build a comprehensive reference of human RNA sequences, isoforms, processing states and chemical modifications...

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rna-seqblog.com /2 weeks ago

Novel biosensor uses DNA ‘barcodes’ to uncover disease signals in cells

RNA sequencing can read Detectron-generated DNA barcodes, enabling scalable detection of specific RNA signals associated with infection, gene activity and other cellular processes....

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journals.plos.org /1 month ago

Characterization of METTL3/14-mediated m<sup>6</sup>A modification in human transcriptome using Nanopore dir...

by Emily Kurtyan, Andrew J. Stein, Kelly J. Abdalla, Zhangerjiao Yuan, Miten Jain, Fadia Ibrahim Post-transcriptional RNA modifications modulate diverse aspects of RNA metabolism....

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rna-seqblog.com /1 month ago

A real-data approach to evaluating RNA transcript analysis methods

RNA sequencing data provide a real-world framework for comparing differential transcript usage methods across bulk, single-cell, spatial, long-read, and short-read transcriptomics....

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rna-seqblog.com /3 weeks ago

Nanopore direct RNA sequencing reveals the challenges of detecting RNA modifications

Direct RNA sequencing with Oxford Nanopore technology enables detection of RNA modifications, but benchmarking reveals substantial differences in accuracy among current computation...

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rna-seqblog.com /1 month ago

New method allows scientists to follow gene activity over time in the same cells

Live-cell RNA sequencing uses engineered virus-like particles to repeatedly sample transcriptomes without destroying cells, enabling researchers to track gene expression changes ov...

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rna-seqblog.com /1 month ago

Benchmarking RNA sequencing for more accurate alternative splicing analysis

RNA sequencing benchmarking across 42 laboratories identifies data quality, sequencing depth, and bioinformatics choices that improve alternative splicing and isoform analysis...

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rna-seqblog.com /1 month ago

Single-cell and single-embryo RNA sequencing

Single-cell and single-embryo RNA sequencing enables detailed analysis of gene expression and cell identity during the earliest stages of embryonic development...

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rna-seqblog.com /1 month ago

RNA variant imaging for cancer diagnosis

RNA Variant Imaging is attracting attention because it addresses a persistent blind spot in cancer diagnostics: many clinically relevant RNA isoforms, splice events, and fusion tra...

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rna-seqblog.com /2 weeks ago

PacBio and TychoBio Collaborate to Train AI Models for Rare Disease

PacBio Kinnex sequencing will generate full-length transcript data to help advance TychoBio’s drug discovery models PacBio, developer of the world’s most advanced sequencing techno...

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rna-seqblog.com /2 days ago

RNA sequencing reveals how genes combine to regulate inflammation and immune responses

Long-read RNA sequencing reveals functional chimeric mRNAs formed from distant genes, identifying a previously unknown protein that regulates inflammation, antibacterial immunity a...

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genengnews.com /2 weeks ago

The Epitranscriptome Heads Toward Clinical Necessity

Short-read RNA sequencing reporting the modifications that alter protein output is starting to make epitranscriptomics indispensable for patient selection in precision medicines. T...

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rna-seqblog.com /1 month ago

POND-seq enables non-destructive RNA sequencing in living cells

POND-seq enables non-destructive RNA sequencing of living cells, allowing repeated measurement of transcriptomes and RNA-binding protein interactions as cells respond to changing c...

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rna-seqblog.com /1 month ago

Detection and sequencing of Ap2N-capped RNAs in human cells

RNA sequencing identifies newly characterized Ap2N caps on human RNAs, including tRNA-derived fragments, revealing additional complexity in RNA processing and responses to oxidativ...

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Sources covering Long Read Sequencing

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journals.plos.org

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journals.plos.org

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rna-seqblog.com

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rna sequencing Publications gene expression Workflow transcriptomics Direct RNA sequencing RNA modifications Nanopore sequencing Single-cell RNA sequencing alternative splicing long-read RNA sequencing Commentary

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