Latest updates for Ribosome Profiling

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Recent items include:

  • RNA sequencing method maps RNA binding proteins with higher precision
  • Mapping bacterial transcription with RNAP-seq
  • A long-read RNA sequencing and polysome profiling framework reveals transposable element-driven transcript diversity

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rna-seqblog.com /1 month ago

RNA sequencing method maps RNA binding proteins with higher precision

RNA sequencing enables precise mapping of RNA binding protein targets, improving detection of low-abundance interactions and advancing understanding of gene regulation...

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rna-seqblog.com /2 weeks ago

Mapping bacterial transcription with RNAP-seq

RNA sequencing method RNAP-seq maps bacterial transcription with single-nucleotide precision, revealing lineage-specific RNA polymerase pausing and regulatory factor...

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rna-seqblog.com /1 month ago

A long-read RNA sequencing and polysome profiling framework reveals transposable element-driven transcript diversity

RNA sequencing reveals how transposable elements drive alternative splicing and alter translation in glioblastoma, generating diverse RNA isoforms linked to cancer progression...

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rna-seqblog.com /1 month ago

SPTEdU-seq – parallel optics-free newborn cell tracking and spatial total transcriptional dynamics

RNA sequencing combined with spatial profiling tracks gene expression and cell proliferation, revealing dynamic cellular interactions and uncovering new insights into development.....

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journals.plos.org /1 week ago

The <i>Pseudomonas aeruginosa</i> ribonuclease Ribocin cleaves eukaryotic ribosomes at helix 69 to inhibit h...

by Alejandro Vasquez-Rifo, Denis Susorov, Emily H. Sholi, Gabriel Demo, Yasaman Jami, Jihui Sha, James A. Wohlschlegel, Andrei Korostelev, Victor Ambros Pseudomonas aeruginosa emp...

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rna-seqblog.com /3 weeks ago

Non-destructive transcriptomics via vesicular export

RNA sequencing with NTVE enables repeated monitoring of gene expression in living cells, supporting time-course analysis of differentiation, perturbation responses, and cellular dy...

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nanowerk.com /1 month ago

Raman spectroscopy maps protein profiles in living cells without destroying them

Researchers used Raman spectroscopy to infer proteome profiles from intact E. coli cells, revealing conserved protein ratio patterns that also appear in human cells.

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journals.plos.org /1 month ago

Dietary restriction shapes intergenerational ribosome abundance and early growth of <i>Caenorhabditis elegans</...

by Sigma Pradhan, Klement Stojanovski, Ferdinand Dellemann, Sacha Psalmon, Joel Tuomaala, Nicholas E. Stroustrup, Benjamin D. Towbin Cells adjust their proteome to their environme...

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rna-seqblog.com /1 week ago

Transcriptomics in space, an RNA sequencing pipeline for space biology research

RNA sequencing supports space biology research by enabling transcriptome-wide analysis of gene expression changes associated with spaceflight and adaptation to space environments.....

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journals.plos.org /1 month ago

The selfish ribosome

by Mart Krupovic, Eugene V. Koonin The ribosome is responsible for protein synthesis in all cells, and is the cell’s largest energy consumer. We propose that the ribosome originat...

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rna-seqblog.com /3 days ago

TAP-seq – targeted single-cell RNA and perturbation sequencing

TAP-seq combines genome editing with targeted RNA sequencing to enable sensitive and cost-effective single-cell functional genomics screens...

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rna-seqblog.com /1 month ago

RNA sequencing moves toward absolute measurement of gene expression

RNA sequencing calibration using standardized references enables absolute quantification of gene expression, improving cross-study comparisons and reducing bias across experiments...

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rna-seqblog.com /1 month ago

New sequencing method exposes hidden gaps in immune signaling

RNA sequencing combined with protein analysis enables detailed tracking of immune cell activity, revealing cytokine dynamics and improving understanding of cancer and immune respon...

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rna-seqblog.com /2 days ago

Decima predicts gene expression at single-cell resolution

RNA sequencing from more than 22 million cells trained a model that predicts cell-type-specific gene expression and noncoding variant...

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rna-seqblog.com /1 month ago

In vivo RNA sequencing reveals real-time immune response to infection

RNA sequencing of newly synthesized transcripts reveals dynamic immune cell activation and gene regulation during infection, providing real-time insight into how immune responses a...

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rna-seqblog.com /1 month ago

FIRST-seq – a nanopore-based cDNA sequencing platform for RNA modification and structure profiling

RNA sequencing using nanopore technology enables detection of RNA modifications and structure at single-nucleotide resolution through reverse transcription error signatures...

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genengnews.com /1 month ago

New Single‑Cell Platform Tracks RNA and Protein in Immune Signaling

CIPHER‑seq is a single‑cell method that measures RNA and proteins simultaneously, exposing gaps in cytokine signaling and reducing mitochondrial stress signatures to give a clearer...

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scienceblog.com /3 weeks ago

New Method Reads a Cell’s Genes Without Killing It

Every few minutes, a living cell does something quietly extraordinary: it sheds tiny membrane bubbles into the fluid around it. These extracellular vesicles carry molecular cargo (...

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rna-seqblog.com /2 weeks ago

Gel-free library preparation for next-generation RNA sequencing and small RNA quantification

RNA sequencing sensitivity improves with gel-free library preparation, reducing primer dimer artifacts and enhancing small RNA quantification from lower sample inputs...

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rna-seqblog.com /2 weeks ago

A*STAR scientists develop new method to reveal how RNA structure influences health and disease

RNA sequencing with the new sm-PORE-cupine method reveals how individual RNA molecules fold and behave, offering insights into gene regulation, viral biology, and therapeutic disco...

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rna-seqblog.com /3 weeks ago

Benchmarking nanopore RNA sequencing for m6A detection

RNA sequencing with nanopore technology improves m6A modification mapping when paired with calibrated workflows, helping reveal cell-specific RNA regulation in fibroblasts and neur...

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journals.plos.org /1 day ago

Type II tRNA cleavage by SLFN14 endoribonuclease variants linked to inherited thrombocytopenia drives global translation...

by Chengchao Ding, Xinyi Ashley Liu, Fushun Zhang, Saori Uematsu, Shu-Bing Qian, Yan Xiang Schlafens proteins (SLFNs) are interferon-inducible regulators of RNA metabolism that in...

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rna-seqblog.com /1 month ago

RNA sequencing without references uncovers hidden transcriptome features

RNA sequencing using reference-free analysis uncovers novel transcripts and hidden gene features, expanding discovery beyond traditional genome-based approaches...

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journals.plos.org /1 month ago

Translation control by altered start codon usage as a means of modulating the general stress response and virulence in &...

by Jialun Wu, Claire Kelly, Duarte N. Guerreiro, Brenda Chanza, Ashley Reade, Catherine M. Burgess, Conor O’Byrne In the food-borne pathogen Listeria monocytogenes, SigB is the ce...

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journals.plos.org

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scienceblog.com

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nanowerk.com

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rna-seqblog.com

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