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rna-seqblog.com /3 weeks ago

Nanopore direct RNA sequencing reveals the challenges of detecting RNA modifications

Direct RNA sequencing with Oxford Nanopore technology enables detection of RNA modifications, but benchmarking reveals substantial differences in accuracy among current computation...

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journals.plos.org /1 month ago

Characterization of METTL3/14-mediated m<sup>6</sup>A modification in human transcriptome using Nanopore dir...

by Emily Kurtyan, Andrew J. Stein, Kelly J. Abdalla, Zhangerjiao Yuan, Miten Jain, Fadia Ibrahim Post-transcriptional RNA modifications modulate diverse aspects of RNA metabolism....

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rna-seqblog.com /1 month ago

Eclipsebio expands its RNA characterization capabilities with multi-attribute nanopore-based sequencing assay eSTRAND RN...

RNA sequencing with eSTRAND RNA QC enables simultaneous measurement of RNA identity, poly(A) tail length, capping efficiency, fragmentation hotspots, and dsRNA...

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genengnews.com /2 weeks ago

Expanding the Possibilities of Next-Generation Sequencing

Sequencing by expansion (SBX) and Roche’s AXELIOS 1 platform introduce a new approach to single-molecule sequencing, designed to give researchers flexibility across sequencing work...

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rna-seqblog.com /1 month ago

Eclipsebio expands its RNA characterization capabilities with multi-attribute nanopore-based sequencing assay eSTRAND RN...

Eclipsebio’s eSTRAND RNA QC uses direct RNA sequencing to measure multiple RNA quality attributes, including identity, poly(A) tails...

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rna-seqblog.com /2 weeks ago

Postdoctoral Fellow – Long-Read RNA Methods Development for Neurodegeneration

The NIH is recruiting a postdoctoral researcher to advance long-read RNA sequencing methods for studying gene regulation, transcript diversity, and RNA modifications in neurodegene...

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rna-seqblog.com /2 weeks ago

Novel biosensor uses DNA ‘barcodes’ to uncover disease signals in cells

RNA sequencing can read Detectron-generated DNA barcodes, enabling scalable detection of specific RNA signals associated with infection, gene activity and other cellular processes....

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deccanchronicle.com /1 week ago

BRIC-CDFD, Oxford Nanopore Launch Genomics Centre

The CoE was jointly inaugurated by Union minister Dr Jitendra Singh and UK minister Jonathan Reynolds during the eighth India-UK Science and Innovation Council meeting in New Delhi...

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rna-seqblog.com /1 month ago

POND-seq enables non-destructive RNA sequencing in living cells

POND-seq enables non-destructive RNA sequencing of living cells, allowing repeated measurement of transcriptomes and RNA-binding protein interactions as cells respond to changing c...

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rna-seqblog.com /3 days ago

“Raman Genome” for SARS-CoV-2: new Raman technology distinguishes viral variants by their molecular RNA architecture

Researchers used Raman spectroscopy to distinguish 20 SARS-CoV-2 variants through RNA structural fingerprints, demonstrating a potential faster alternative to RNA sequencing...

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rna-seqblog.com /1 month ago

Detection and sequencing of Ap2N-capped RNAs in human cells

RNA sequencing identifies newly characterized Ap2N caps on human RNAs, including tRNA-derived fragments, revealing additional complexity in RNA processing and responses to oxidativ...

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rna-seqblog.com /2 weeks ago

How should biological replicates be handled in long-read RNA sequencing?

Long-read RNA sequencing analysis shows that handling biological replicates differently can influence transcript reconstruction and the discovery of novel RNA isoforms...

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rna-seqblog.com /1 month ago

An end-to-end computational framework for “Record-seq” transcriptional recording data

A new framework adapts RNA sequencing analysis for Record-seq, improving spacer extraction, transcription-unit quantification, normalization, differential expression, and modeling...

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nanowerk.com /1 month ago

Stress-driven nanopore method could simplify biosensor manufacturing

A stress-driven fabrication method creates 6–10 nm nanopores in parallel, potentially shifting biosensor production to conventional semiconductor fabs.

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rna-seqblog.com /2 weeks ago

PacBio and TychoBio Collaborate to Train AI Models for Rare Disease

PacBio Kinnex sequencing will generate full-length transcript data to help advance TychoBio’s drug discovery models PacBio, developer of the world’s most advanced sequencing techno...

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rna-seqblog.com /1 month ago

New method allows scientists to follow gene activity over time in the same cells

Live-cell RNA sequencing uses engineered virus-like particles to repeatedly sample transcriptomes without destroying cells, enabling researchers to track gene expression changes ov...

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rna-seqblog.com /3 weeks ago

The Human RNome Project Consortium seeks to publish first-ever complete sequence of the bodyÐ²Ð‚â„¢s RNA

RNA sequencing efforts from the Human RNome Project aim to build a comprehensive reference of human RNA sequences, isoforms, processing states and chemical modifications...

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rna-seqblog.com /1 month ago

Ultrafast and reference-free sequence discovery in single-cell data

Malva enables ultrafast, reference-free searches across massive single-cell RNA sequencing datasets for mutations, splice junctions, pathogens, transcripts, and other sequence-leve...

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genengnews.com /1 week ago

Thirty Million Compartments: Testing Single-Molecule PCR’s Potential

Countable Labs has named Fred Hutchinson its first certified service provider, currently serving researchers across the Seattle region. And the company is continuing to build its l...

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journals.plos.org /1 month ago

Direct microhaplotype genotyping for GT-seq (Genotyping-in-Thousands by Sequencing) using a diploid abundance model

by Nathan R. Campbell, Amanda R. Campbell, Shannon K. Blair, Amanda J. Finger GT-seq (Genotyping-in-Thousands by Sequencing) is widely used for high-throughput amplicon genotyping...

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journals.plos.org /1 month ago

Hunting for microsatellite instability in long-read data with Owl

by Zev Kronenberg, Byunggil Yoo, Khi Pin Chua, Mark J. P. Chaisson, Lisa Lansdon, William J. Rowell, Guilherme de Sena Brandine, Jocelyne Bruand, Egor Dolzhenko, Kobe Ikegami, Jay...

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food-safety.com /5 days ago

Whole Genome Sequencing in Food Safety: Non-Technical Systems Barriers and Opportunities for Enhanced Global Use

Whole genome sequencing is a mature, reliable technology with significant potential for the food industry, but realizing its benefits requires organizations to strategically weigh...

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rna-seqblog.com /1 month ago

Benchmarking RNA sequencing for more accurate alternative splicing analysis

RNA sequencing benchmarking across 42 laboratories identifies data quality, sequencing depth, and bioinformatics choices that improve alternative splicing and isoform analysis...

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rna-seqblog.com /2 weeks ago

DeCoRE – a computational method to resolve RNA structural heterogeneity from RNA structure probing data by direct RNA se...

Direct RNA sequencing with DeCoRE reveals alternative RNA structures and folding dynamics at single-nucleotide resolution, providing a detailed view of transcript-level structural....

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