Latest updates for Sequencing Protocols

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Recent items include:

  • Integrated Sample Preparation System Standardizes and Streamlines Pre-Analytical Workflows
  • Gel-free library preparation for next-generation RNA sequencing and small RNA quantification
  • BINSEQ: A family of high-performance binary formats for nucleotide sequences

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genengnews.com /1 month ago

Integrated Sample Preparation System Standardizes and Streamlines Pre-Analytical Workflows

The Invivoscribe PrepQuant system is assay agnostic, generating highly concentrated genomic DNA and cell free DNA (cfDNA) yields for next-generation sequencing (NGS), qPCR, and dig...

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rna-seqblog.com /2 weeks ago

Gel-free library preparation for next-generation RNA sequencing and small RNA quantification

RNA sequencing sensitivity improves with gel-free library preparation, reducing primer dimer artifacts and enhancing small RNA quantification from lower sample inputs...

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journals.plos.org /2 days ago

BINSEQ: A family of high-performance binary formats for nucleotide sequences

by Noam Teyssier, Alexander Dobin Modern genomics produces billions of sequencing records per run, which are typically stored as gzip-compressed FASTQ files. While this format is...

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genengnews.com /1 month ago

Advancing Fully Walkaway Automation in Genomics Workflows

EMBL GeneCore plans to expand facility capacity to develop new protocols and further validate and optimize existing workflows for applications such as low-input and metagenomics sa...

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food-safety.com /1 month ago

USDA-FSIS Updates WGS Laboratory Method

The updated Microbiology Laboratory Guidebook chapter on whole genome sequencing (WGS) of bacterial isolates now includes protocols for using both the Illumina MiSeq and MiSeq i100...

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journals.plos.org /1 month ago

STARCall integrates image stitching, alignment, and read calling to enable scalable analysis of <i>in situ</i&g...

by Nicholas J. Bradley, Sriram Pendyala, Katie Partington, Douglas M. Fowler Fluorescent in situ sequencing involves imaging-based sequencing by synthesis in intact cells or tissu...

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rna-seqblog.com /1 month ago

CHOP researchers develop versatile and low-cost technology for targeted long-read RNA sequencing

Long-read RNA sequencing improves rare disease diagnosis by resolving transcript isoforms and variant effects, enabling more accurate interpretation of pathogenic splicing...

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rna-seqblog.com /1 month ago

FIRST-seq – a nanopore-based cDNA sequencing platform for RNA modification and structure profiling

RNA sequencing using nanopore technology enables detection of RNA modifications and structure at single-nucleotide resolution through reverse transcription error signatures...

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journals.plos.org /1 month ago

QoALa: A comprehensive workflow for viral quasispecies diversity comparison using long-read sequencing data

by Nakarin Pamornchainavakul, Declan C. Schroeder, Kimberly VanderWaal The concept of viral quasispecies refers to a constantly mutating viral population occurring within hosts, w...

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rna-seqblog.com /3 days ago

TAP-seq – targeted single-cell RNA and perturbation sequencing

TAP-seq combines genome editing with targeted RNA sequencing to enable sensitive and cost-effective single-cell functional genomics screens...

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rna-seqblog.com /1 month ago

Programmable RNA detection enables multiplex virus identification

RNA sequencing advances support CRISPR-based detection methods that use kinetic signatures to identify multiple viruses and variants from RNA in a single, rapid diagnostic assay...

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rna-seqblog.com /1 month ago

RNA sequencing moves toward absolute measurement of gene expression

RNA sequencing calibration using standardized references enables absolute quantification of gene expression, improving cross-study comparisons and reducing bias across experiments...

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journals.plos.org /1 week ago

PowerNovo2: A generative flow-based approach to non-autoregressive <i>de novo</i> peptide sequencing

by Denis V. Petrovskiy, Kirill S. Nikolsky, Vladimir R. Rudnev, Liudmila I. Kulikova, Tatiana V. Butkova, Kristina A. Malsagova, Arthur T. Kopylov, Anna L. Kaysheva Proteomics uti...

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journals.plos.org /1 day ago

Fully synthetic replication of complex real biological cell clusters using a novel cluster-based ‘Rosetta-Routine’ compu...

by Bradley Mason, Laura Justham, Liam Whitby, Alison Whitby, Stuart Scott, Samuel Nti, Jon Petzing Flow cytometry (FC) is essential for the precise quantification and characterisa...

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rna-seqblog.com /1 month ago

SPTEdU-seq – parallel optics-free newborn cell tracking and spatial total transcriptional dynamics

RNA sequencing combined with spatial profiling tracks gene expression and cell proliferation, revealing dynamic cellular interactions and uncovering new insights into development.....

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rna-seqblog.com /1 month ago

Automating RNA sequencing analysis with AutoRNAseq

RNA sequencing workflows like AutoRNAseq automate data processing, improving reproducibility and consistency across experiments while simplifying gene expression analysis for large...

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rna-seqblog.com /2 weeks ago

Mapping bacterial transcription with RNAP-seq

RNA sequencing method RNAP-seq maps bacterial transcription with single-nucleotide precision, revealing lineage-specific RNA polymerase pausing and regulatory factor...

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rna-seqblog.com /1 month ago

Next-generation sequencing has had a huge impact on molecular pathology lab

RNA sequencing and next generation sequencing expand cancer diagnostics by enabling multi gene analysis, improving mutation detection, and supporting targeted therapy decisions in...

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food-safety.com

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journals.plos.org

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rna-seqblog.com

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