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journals.plos.org /1 month ago

Double-CRISPR Knockout Simulation (DKOsim): A Monte-Carlo randomization system to model cell growth behavior and infer t...

by Yue Gu, Traver Hart, Luis Leon-Novelo, John Paul Shen Advances in functional genomic technology, notably CRISPR using Cas9 or Cas12, now allow for large-scale double perturbati...

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genengnews.com /1 month ago

CRISPR Screens Map Human T‑Cell Genes That Promote or Block HIV Infection

Using genome‑wide CRISPR activation and knockout screens in primary human T cells, researchers mapped the human genes that shape HIV infection and identified potent antiviral facto...

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genengnews.com /3 weeks ago

Optical Pooled CRISPR Screen Reveals Regulators of NF-κB Dynamics in Human Cells

In this GEN webinar, Tilmann Buerckstuemmer, PhD, CSO at Myllia Biotechnology will show how high-throughput pooled CRISPR screening combined with cell painting readouts characteriz...

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rna-seqblog.com /3 days ago

TAP-seq – targeted single-cell RNA and perturbation sequencing

TAP-seq combines genome editing with targeted RNA sequencing to enable sensitive and cost-effective single-cell functional genomics screens...

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news-medical.net /1 month ago

Non-clinical safety assessment crucial for CRISPR-based gene therapies

The rapid evolution of CRISPR/Cas genome editing has redefined the possibilities of cellular and gene therapy, enabling precise correction, disruption, and regulation of disease-as...

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bioengineer.org /4 weeks ago

Programmable RNA Targeting via DNA-Guided CRISPR-Cas12a

In a landmark advancement that could redefine molecular diagnostics, researchers have unveiled a groundbreaking DNA-guided CRISPR–Cas12a system capable of programmable RNA recognit...

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rna-seqblog.com /1 month ago

Programmable RNA detection enables multiplex virus identification

RNA sequencing advances support CRISPR-based detection methods that use kinetic signatures to identify multiple viruses and variants from RNA in a single, rapid diagnostic assay...

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journals.plos.org /1 week ago

Cas9-expressing HC-04 hepatocytes facilitate CRISPR-based analysis of <i>Plasmodium falciparum</i> sporozoit...

by Lisa H. Verzier, Eva Hesping, Marcel Doerflinger, Marco J. Herold, Justin A. Boddey Sporozoites of Plasmodium falciparum, the deadliest malaria parasite, are injected into the...

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koreatimes.co.kr /1 month ago

KAIST develops medical tool to identify multiple viruses simultaneously

Scientists at the Korea Advanced Institute of Science and Technology (KAIST), in collaboration with researchers at the University of California, Berkeley and the Gladstone Institut...

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labiotech.eu /1 month ago

Modified CRISPR tool targets Down syndrome mutation

Researchers have used modified CRISPR to silence the extra chromosome 21 in Down syndrome cells, showing promising preclinical results. The post Modified CRISPR tool targets Down s...

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nanowerk.com /1 month ago

CRISPR variant selectively targets tumor DNA

New gene-editing techniques exploit unique chemical fingerprints in cancer DNA to selectively destroy tumor cells, paving the way for precision therapy.

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nanowerk.com /3 weeks ago

New kind of CRISPR could treat viral infection and cancer by shredding sick cells' DNA

New CRISPR tool shreds targeted cells instead of editing genes, showing promise for killing diseased cells while sparing healthy ones.

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genengnews.com /2 weeks ago

DNA‑Guided CRISPR Suggests a New Direction for RNA Editing

The platform, called ΨDNA, reprograms Cas12 nucleases to recognize and act on RNA using a DNA-based guide scaffold. In human cell lines, ΨDNA achieved 70–95% knockdown of endogenou...

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genengnews.com /1 month ago

New Single‑Cell Platform Tracks RNA and Protein in Immune Signaling

CIPHER‑seq is a single‑cell method that measures RNA and proteins simultaneously, exposing gaps in cytokine signaling and reducing mitochondrial stress signatures to give a clearer...

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genengnews.com /4 weeks ago

Gene Editing at Scale, Clinic Seeks Generalizable Therapies

What’s most exciting about CRISPR is its potential to shift medicine from managing disease to directly correcting its root cause. End-to-end pipelines and regulatory advances aim t...

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bioengineer.org /3 weeks ago

HKUST Researchers Pioneer World’s First DNA-Guided Gene Editing Tool, Revolutionizing Infectious Disease Diagnosis and A...

In a remarkable leap forward in gene editing technology, researchers at The Hong Kong University of Science and Technology (HKUST) have developed the world’s first DNA-guided CRISP...

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rna-seqblog.com /1 month ago

MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy

RNA sequencing reveals how nuclear genes regulate mitochondrial DNA copy number and heteroplasmy, uncovering stress responses and potential therapeutic targets for mitochondrial...

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lifespan.io /1 week ago

CRISPR-Based System Targets RNA and Kills Cells on Demand

Scientists have devised a CRISPR-based tool that can kill cells carrying a specific strand of RNA. The tested targets include cancerous and virus-infected cells [1]. Targeted assas...

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genengnews.com /2 weeks ago

Acute Myeloid Leukemia Therapy Improved by CRISPR Stem Cell Transplant

A Phase I/II clinical trial shows that a stem cell transplant that removes CD33 from donor cells using CRISPR can prevent cancer recurrence. The post Acute Myeloid Leukemia Therapy...

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genengnews.com /1 month ago

Epigenetic Strategy Restores Tumor Suppressor in Acute Myeloid Leukemia Models

By combining CRISPR screening with epigenetic targeting, scientists identified a group of enzymes that could be inhibited to restore tumor suppressor activity and reduce leukemia b...

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bioengineer.org /2 weeks ago

CRISPR Technology Shows Promise in Inhibiting Hepatitis E Virus

In a groundbreaking development that could redefine antiviral therapy, researchers have engineered a CRISPR-Cas13d based system to combat hepatitis E virus (HEV) infection with rem...

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journals.plos.org /1 month ago

High-throughput characterization of <i>Mycobacterium tuberculosis</i> gene function across diverse condition...

by Kayla M. Dinshaw, Katie A. Lien, Matthew Knight, Sorel V. Yigma Ouonkap, Hualan Liu, David F. Savage, Hans K. Carlson, Adam M. Deutschbauer, Sarah A. Stanley Mycobacterium tube...

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genengnews.com /1 month ago

Machine Learning and Single-Cell Technology Combined to Drive High-Performance Cell Line Development

The collaboration brings together OneCyte's proprietary single-cell platform for high-throughput and high-speed clone selection with Kemp Proteins' molecular engineering capabiliti...

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bioengineer.org /3 weeks ago

SNU Professor Sangwoo Seo’s Team Develops Next-Generation CRISPR Biocontainment Technology to Control Microbial Survival...

In a groundbreaking advancement at the intersection of genome engineering and biocontainment, researchers at Seoul National University have developed a pioneering technology enabli...

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